Festival and Experiment ; Phospholipid extraction

Posted on Nov 30, 2014 by Unknown

BUABAN Parichat

PARE26-029

Kasetsart University

Loi Krathong festival

When 8^th November 2014, about 3 week ago at Clark memorial student center in Hokkaido university, Thai and Japanese people came to join together. We jointiy held Loi Krathong festival. As well as the my friend and I participated Loi Krathong festival also and we showed Ram Thai.

Photo by Awassada Putchakarm

Loi Krathong festival is an important day for traditional Thai festival. The festival is held in November every day at the moment that we could see full moon on the sky. Normally activities are held on the every area near the river in the country. Those is believe for misfortunes to floating and an worship and ask for a goddess. For this festival Thai and Japanese people had met, talked and made thai food together such as Somtum (green papaya salad) with chicken fried, Yum Woon Sen (spicy glass noodle salad) and other. The shows are Ram Thai and Ramwong (Dance in Thai) including Bingo game rewarded for the winner.

Experiment ; Phospholipid extraction

This is my first experiment in Plant Nutrition laboratory. My staff is Eriko Maejima. She is studying in doctoral degree and teach me about all experiment. We study on the aluminum (Al) tolerance of rice and barley and their lipid composition in roots. Usually rice is more tolerant to Al than other crops. Then, I have been assigned the experiment also phospholipid and sterol determination include screening of aluminum tolerance. Someone asked me, Why I have to study about phospholipid? I therefore explained that because acid soils is the most serious problem for crop product influence to stress in the rhizosphere and limits plant growth by excessive Al availability. Al toxicity has inhibition of root elongation. In root cell has phospholipid and sterols .These are part of plasma membrane(PM) component. When Al binding to negative sites of PM were occur increase the PM permeability. Organic acid anion released from roots is a major Al exclusion mechanism in plant spicies. Therefore, In the PM, Al induced increase in PM permeability can be more severe with a high proportion of phospholipid. That come of hypothesis in this study are in the PM have a higher phospholipid proportion in plant of Al sensitive and in contract if a lower phospholipid proportion in plant of Al tolerant.

In phospholipid extraction. Root sample is homogenized three time in extraction solution are 2-propanol, chloroform and milliQ water.

Extraction solution with a mortar and pestle.

Collect supernatant and all residue into grass tube. The homogenized sample is centrifuged for the chloroform layer separated and collected the layer into flask for three time. After that into seprating funnel for extraction with solution mixture (chloroform : 2-propanol: 0.1 M KCl) and shaken with the same volumn 0.1 M KCl several time .